"Effect of Short- and Long-Term High-Fat Feeding on Autophagy Flux and Lysosomal Activity in Rat Liver"
HF-2 and HF-10 are 2 and 10 week high fat diets, where fat was w.w. 33% (99% lard + 1% soybean oil), protein 14%, starch 17%, vs SD which was protein 22%, fat 3%, starch 68%.
The HF-10 rats developed hyperinsulinemia, but not SD/HF-2. I'm interested whether high fat diet has similar effect in humans.
Here's the observation of the authors:
We found no difference in body weight among
experimental groups (Table 3). The adiposity (expressed
as weight of epididymal fat pad per 100 grams body
weight) was similar in SD and HF-2 but significantly
higher in HF-10. Fasting glycaemia was elevated in both
HF-2 and HF-10 groups but hyperinsulinemia was proved
only in HF-10. The alterations in glucose metabolism
were not accompanied by fasting hypertriglyceridemia.
The increased fat utilisation for energy production was
documented by the elevation of β-hydroxybutyrate
content in serum in HF-2 and HF-10 animals.
Interestingly, ketogenesis in fasting is lower in HF-10
compared with HF-2 group (P=0.035) which suggests a
tendency to the attenuation of TAG utilisation in the
liver. As expected, compared with the SD group, the HF
diet administered animals accumulated an increased
amount of TAG in the liver depending on the length of
diet administration.